| 1 | –The Stanley Brothers | Molly And TenbrooksWritten-By – B. Monroe | 2:26 |
| 2 | –Bill Monroe | Bluegrass BreakdownWritten-By – B. Monroe | 2:19 |
| 3 | –The Stanley Brothers | I'm A Man Of Constant SorrowArranged By – C. StanleyWritten-By – Trad. | 2:58 |
| 4 | –Sonny Osborne | The Old Cross RoadWritten-By – B. Monroe | 2:42 |
| 5 | –The Stanley Brothers | Little MaggieWritten-By – Trad. | 2:19 |
| 6 | –The Lilly Brothers | They Sleep Together Now At RestWritten-By – Trad. | 3:11 |
| 7 | –The Lonesome Pine Fiddlers | You Broke Your PromiseWritten-By – C. Cline, P. Williams | 2:09 |
| 8 | –Wilma Lee & Stoney Cooper | This World Can't Stand LongWritten-By – R. Acuff | 2:37 |
| 9 | –Jimmy Martin & The Osborne Brothers | Across The Sea BluesWritten-By – J. Martin | 3:16 |
| 10 | –Mac Wiseman | I Wonder How The Old Folks Are At HomeWritten-By – Trad. | 2:50 |
| 11 | –Mac Wiseman | Dreaming Of A Little CabinWritten-By – A. Brumley | 2:55 |
| 12 | –Bill Monroe | Blue Moon Of KentuckyWritten-By – B. Monroe | 3:02 |
| 13 | –Flatt & Scruggs | My Cabin In CarolineWritten-By – L. Flatt/E. Scruggs | 2:38 |
| 14 | –Mac Wiseman | Little White ChurchWritten-By – E. Wellman | 2:37 |
| 15 | –Reno & Smiley | I'm Using My Bible For A RoadmapWritten-By – C. Schroeder, D. Reno | 2:32 |
| 16 | –Sonny Osborne | Get In Line BrotherWritten-By – L. Flatt | 2:42 |
| 17 | –The Lonesome Pine Fiddlers | Pain In My HeartWritten-By – B. Osborne, Richardson | 2:42 |
| 18 | –Bill Monroe | Uncle PenWritten-By – B. Monroe | 2:45 |
| 19 | –The Lonesome Pine Fiddlers | Will I Meet My Brother In HeavenWritten-By – B. Osborne, L. Richardson | 2:18 |
| 20 | –Brown's Ferry Four | Will The Circle Be UnbrokenWritten-By – Trad. | 2:56 |
| 21 | –The Stanley Brothers | Little Glass Of WineWritten-By – C. Stanley | 2:56 |
| 22 | –Sonny Osborne | I'm Gonna Leave YouWritten-By – Trad. | 2:39 |
| 23 | –Brown's Ferry Four | Over In The Glory LandWritten-By – Trad. | 1:59 |
| 24 | –The Sauceman Brothers | Hallelujah We Shall RiseWritten-By – Trad. | 1:54 |
| 25 | –Flatt & Scruggs | Foggy Mountain BreakdownWritten-By – E. Scruggs | 2:41 |
| 26 | –Wilma Lee & Stoney Cooper | Wicked Path Of SinWritten-By – B. Monroe | 2:31 |
| 27 | –Bill Monroe | Footprints In The SnowArranged By – B. LaneWritten-By – Trad. | 2:39 |
| 28 | –Sonny Osborne | The Old Home TownWritten-By – L. Flatt | 2:47 |
| 29 | –Flatt & Scruggs | Old Salty Dog BluesWritten-By – W. Morris, Z. Morris | 2:30 |
| 30 | –Mac Wiseman | Going To See My BabyWritten-By – A. Smith | 2:27 |
| 31 | –The Lonesome Pine Fiddlers | Nobody Cares (Not Even You)Written-By – C. Cline, P. Williams | 2:20 |
| 32 | –Frank Hunter & His Black Mountain Boys | Tennessee BoyWritten-By – F. Hunter | 3:16 |