| 1 | –Mississippi John Hurt | Stack O' Lee Blues | 2:45 |
| 2 | –Butterbeans And Susie | What It Takes To Bring You Back (Mama Keeps It All The Time) | 3:24 |
| 3 | –Texas Alexander | Broken Yo-Yo | 3:02 |
| 4 | –Leadbelly | Pig Meat Papa | 3:15 |
| 5 | –Elmore James | Sunnyland | 2:20 |
| 6 | Blues And Entertainment |
| 7 | –Peetie Wheatstraw | Good Whiskey Blues | 3:08 |
| 8 | –Robert Johnson | Little Queen Of Spades | 2:14 |
| 9 | –Lillian Glinn | Shake It Down | 3:12 |
| 10 | –Joe Williams And Sonny Boy Williamson | Wild Cow Moan | 2:51 |
| 11 | The Thirties, Urban And Rural Blues |
| 12 | –Big Bill Broonzy | All By Myself | 2:26 |
| 13 | –Casey Bill And Black Bob | W.P.A. Blues | 3:15 |
| 14 | –Charley Patton | Stone Pony Blues | 2:49 |
| 15 | –Barbecue Bob And Laughing Charley | It Won't Be Long Now - (Part One) | 3:28 |
| 16 | –Mississippi Jook Band | Dangerous Woman | 2:43 |
| 17 | –Otis Spann | Bloody Murder | 3:34 |
| 18 | –Blind Lemon Jefferson | Black Snake Moan | 3:01 |
| 19 | –Bukka White | Parchman Farm Blues | 2:37 |
| 20 | –Bo Carter | Sorry Feeling Blues | 3:08 |
| 21 | World War Ⅱ And After |
| 22 | –Fra-Fra Tribesmen | Yarum Praise Songs | 2:56 |
| 23 | –Big Joe Turner And Pete Johnson | Roll 'Em Pete | 2:50 |
| 24 | –Leroy Carr And Scrapper Blackwell | Midnight Hour Blues | 3:04 |
| 25 | –Memphis Minnie | Me And My Chauffeur Blues | 2:45 |
| 26 | –Blind Willie McTell | Travelin' Blues | 3:18 |
| 27 | –Henry Williams And Eddie Anthony | Georgia Crawl | 3:20 |
| 28 | –Bessie Smith | In The House Blues | 3:00 |
| 29 | –Memphis Jug Band | Gator Wobble | 2:39 |
| 30 | –Bertha "Chippie" Hill | Pratt City Blues | 2:53 |
| 31 | The Origins Of The Blues |
| 32 | –Brownie McGhee | Million Lonesome Women | 2:34 |
| 33 | –Faber Smith And Jimmy Yancey | East St. Louis Blues | 2:48 |
| 34 | –Blind Boy Fuller And Sonny Terry | I Want Some Of Your Pie | 2:40 |
| 35 | –Johnny Shines | I Don't Know | 3:30 |
| 36 | –Peg Leg Howell | Broke And Hungry Blues | 3:19 |